Analysis of Common Ingredients
in Cold Medicines 
using HPLC-UV

Analysis of Ingredients in Cold Medicines using HPLC-UV


  1. Acetaminophen (0.13 mg/mL)
  2. Caffeine (0.03 mg/mL)
  3. Salicylamide (0.14 mg/mL)
  4. Aspirin (0.58 mg/mL)
  5. Ethenzamide (0.28 mg/mL)

HPLC Analytical Conditions:

Column: Develosil HSR C18, 5 µm ( 4.6 x 150 mm)
Mobile Phase:Methanol/50 mM NH₄H₂PO₄ (pH=2.5) = 40/60
Flow Rate:1.0 mL/min
Detection:UV at 280 nm
Injection Volume:5.0 µL

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